Long-term exposure to Porphyromonas gingivalis causes inflammation and degeneration of brain neurons in mice that is similar to the effects of Alzheimer’s disease in human beings, according to the University of Illinois at Chicago (UIC). These findings suggest that periodontal disease may be an initiator of Alzheimer’s disease, researchers there report.
“Other studies have demonstrated a close association between periodontitis and cognitive impairment, but this is the first study to show that exposure to the periodontal bacteria results in the formation of senile plaques that accelerate the development of neuropathology found in Alzheimer’s patients,” said Keiko Watanabe, DDS, professor of periodontics at the UIC College of Dentistry and corresponding author of the study.
“This was a big surprise,” Watanabe said. “We did not expect that the periodontal pathogen would have this much influence on the brain or that the effects would so thoroughly resemble Alzheimer’s disease.”
To study the impact of the bacteria on brain health, the researchers established chronic periodontitis in 10 wild-type mice. Another 10 mice served as the control group. After 22 weeks of repeated oral application of the bacteria to the study group, the researchers studied the brain tissue of the mice and compared brain health.
The mice chronically exposed to the bacteria had significantly higher amounts of accumulated amyloid beta, a senile plaque found in the brain tissue of Alzheimer’s patients. The study group also had more brain inflammation and fewer intact neurons due to degeneration.
These findings were further supported by amyloid beta protein analysis and RNA analysis that showed greater expression of genes associated with inflammation and degeneration in the study group. DNA from the periodontal bacteria was also found in the brain tissue of mice in the study group, and a bacterial protein was observed inside their neurons.
“Our data not only demonstrate the movement of bacteria from the mouth to the brain, but also that chronic infection leads to neural effects similar to Alzheimer’s,” Watanabe said.
The researchers say these findings are powerful in part because they used a wild-type mouse model. Most model systems used to study Alzheimer’s rely on transgenic mice, which have been genetically altered to more strongly express genes associated with the senile plaque and enable Alzheimer’s development.
“Using a wild-type mouse model added strength to our study because these mice were not primed to develop the disease, and use of this model gives additional weight to our findings that periodontal bacteria may kickstart the development of the Alzheimer’s,” Watanabe said.
The researchers say that understanding causality and risk factors for the development of Alzheimer’s is critical to the development of treatments, particularly when it comes to sporadic or late-onset disease, which constitutes more than 95% of cases and has largely unknown causes and mechanisms. Watanabe also said the findings offer lessons for everyone.
“Oral hygiene is an important predictor of disease, including diseases that happen outside the mouth,” she said. “People can do so much more for their personal health by taking oral health seriously.”
The study, “Chronic Oral Application of a Periodontal Pathogen Results in Brain Inflammation, Neurodegeneration and Amyloid Beta Production in Wild Type Mice,” was published by PLOS One.
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