The goal of the OHSU study was to use a light-generating gene for development of an oral bacteria biosensor, and then examine the effect of minocycline hydrochloride either alone or in combination with lidocaine/prilocaine or chlorhexidine. The OHSU research team found that minocycline hydrochloride produced quick and effective results in treating periodontal infection. When lidocaine and prilocaine were added to the minocycline hydrochloride regimen, there was no interference with the potent antibacteriostatic activity of minocycline hydrochloride, but actually an additive antibacterial effect. The chlorhexidine rinse, in conjunction with either high or low doses of minocycline hydrochloride, also had an additive effect.
“We were pleased at the sensitivity of the bioluminescence-generating lux gene in the bacterial transformants and its use in determining antimicrobial activity. The biosensor system also allowed us to rapidly monitor inhibition of bacterial metabolism, which is promising,” noted Curt Machida, PhD, principal investigator and professor of integrative biosciences and pediatric dentistry in the OHSU School of Dentistry. Dr. Machida’s lab also hosted the study.
(Source: OHSU School of Dentistry, October 13, 2010)